RapamycinFungusV1, Main, Exploration, bibRecord, 000707

The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals.

Identifieur interne : 000707 ( Main/Exploration ); précédent : 000706; suivant : 000708

The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals.

Auteurs : Gabriel Talaia [Portugal] ; Christos Gournas [Belgique] ; Elie Saliba [Belgique] ; Cláudia Barata-Antunes [Portugal] ; Margarida Casal [Portugal] ; Bruno André [Belgique] ; George Diallinas [Grèce] ; Sandra Paiva [Portugal]

Source :

Journal of molecular biology [ 1089-8638 ] ; 2017.

RBID : pubmed:28965784

Descripteurs français

KwdFr :
- Alcalis (pharmacologie), Arrestine (métabolisme), Endocytose (effets des médicaments et des substances chimiques), Endocytose (physiologie), Membrane cellulaire (métabolisme), Protein Phosphatase 2 (métabolisme), Protein kinases (métabolisme), Protéines de Saccharomyces cerevisiae (métabolisme), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (métabolisme), Symporteurs (métabolisme), Transduction du signal (effets des médicaments et des substances chimiques), Transport biologique (MeSH), Transporteurs d'acides monocarboxyliques (métabolisme), Ubiquitin-protein ligases (métabolisme), Voies et réseaux métaboliques (effets des médicaments et des substances chimiques).
MESH :
- croissance et développement : Saccharomyces cerevisiae.
- effets des médicaments et des substances chimiques : Endocytose, Transduction du signal, Voies et réseaux métaboliques.
- métabolisme : Arrestine, Membrane cellulaire, Protein Phosphatase 2, Protein kinases, Protéines de Saccharomyces cerevisiae, Saccharomyces cerevisiae, Symporteurs, Transporteurs d'acides monocarboxyliques, Ubiquitin-protein ligases.
- pharmacologie : Alcalis.
- physiologie : Endocytose.
- Transport biologique.

English descriptors

KwdEn :
- Alkalies (pharmacology), Arrestin (metabolism), Biological Transport (MeSH), Cell Membrane (metabolism), Endocytosis (drug effects), Endocytosis (physiology), Metabolic Networks and Pathways (drug effects), Monocarboxylic Acid Transporters (metabolism), Protein Kinases (metabolism), Protein Phosphatase 2 (metabolism), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (metabolism), Signal Transduction (drug effects), Symporters (metabolism), Ubiquitin-Protein Ligases (metabolism).
MESH :
- chemical , metabolism : Arrestin, Monocarboxylic Acid Transporters, Protein Kinases, Protein Phosphatase 2, Saccharomyces cerevisiae Proteins, Symporters, Ubiquitin-Protein Ligases.
- chemical , pharmacology : Alkalies.
- drug effects : Endocytosis, Metabolic Networks and Pathways, Signal Transduction.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Cell Membrane, Saccharomyces cerevisiae.
- physiology : Endocytosis.
- Biological Transport.

Abstract

Eukaryotic α-arrestins connect environmental or stress signaling pathways to the endocytosis of plasma membrane transporters or receptors. The Saccharomyces cerevisiae lactate transporter Jen1p has been used as a model cargo for elucidating the mechanisms underlying endocytic turnover in response to carbon sources. Here, we discover a novel pathway of Jen1p endocytosis mediated by the α-arrestin Bul1p in response to the presence of cycloheximide or rapamycin, or prolonged growth in lactate. While cycloheximide or rapamycin modify cells pleiotropically, the major effect of prolonged growth in lactate was shown to be external pH alkalinization. Importantly, employment of specific inactive Jen1p versions showed that Bul1p-dependent endocytosis requires lactate transport, according to the signal imposed. Our results support a model where conformational changes of Jen1p, associated with substrate/H⁺ symport, are critical for the efficiency of Bul1p-dependent Jen1p turnover.

DOI: 10.1016/j.jmb.2017.09.014
PubMed: 28965784

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkalies (pharmacology)</term>
<term>Arrestin (metabolism)</term>
<term>Biological Transport (MeSH)</term>
<term>Cell Membrane (metabolism)</term>
<term>Endocytosis (drug effects)</term>
<term>Endocytosis (physiology)</term>
<term>Metabolic Networks and Pathways (drug effects)</term>
<term>Monocarboxylic Acid Transporters (metabolism)</term>
<term>Protein Kinases (metabolism)</term>
<term>Protein Phosphatase 2 (metabolism)</term>
<term>Saccharomyces cerevisiae (growth & development)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
<term>Signal Transduction (drug effects)</term>
<term>Symporters (metabolism)</term>
<term>Ubiquitin-Protein Ligases (metabolism)</term>
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<term>Arrestine (métabolisme)</term>
<term>Endocytose (effets des médicaments et des substances chimiques)</term>
<term>Endocytose (physiologie)</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Protein Phosphatase 2 (métabolisme)</term>
<term>Protein kinases (métabolisme)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Saccharomyces cerevisiae (croissance et développement)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Symporteurs (métabolisme)</term>
<term>Transduction du signal (effets des médicaments et des substances chimiques)</term>
<term>Transport biologique (MeSH)</term>
<term>Transporteurs d'acides monocarboxyliques (métabolisme)</term>
<term>Ubiquitin-protein ligases (métabolisme)</term>
<term>Voies et réseaux métaboliques (effets des médicaments et des substances chimiques)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arrestin</term>
<term>Monocarboxylic Acid Transporters</term>
<term>Protein Kinases</term>
<term>Protein Phosphatase 2</term>
<term>Saccharomyces cerevisiae Proteins</term>
<term>Symporters</term>
<term>Ubiquitin-Protein Ligases</term>
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</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Endocytosis</term>
<term>Metabolic Networks and Pathways</term>
<term>Signal Transduction</term>
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<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Endocytose</term>
<term>Transduction du signal</term>
<term>Voies et réseaux métaboliques</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arrestine</term>
<term>Membrane cellulaire</term>
<term>Protein Phosphatase 2</term>
<term>Protein kinases</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae</term>
<term>Symporteurs</term>
<term>Transporteurs d'acides monocarboxyliques</term>
<term>Ubiquitin-protein ligases</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alcalis</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Endocytosis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Transport biologique</term>
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<front><div type="abstract" xml:lang="en">Eukaryotic α-arrestins connect environmental or stress signaling pathways to the endocytosis of plasma membrane transporters or receptors. The Saccharomyces cerevisiae lactate transporter Jen1p has been used as a model cargo for elucidating the mechanisms underlying endocytic turnover in response to carbon sources. Here, we discover a novel pathway of Jen1p endocytosis mediated by the α-arrestin Bul1p in response to the presence of cycloheximide or rapamycin, or prolonged growth in lactate. While cycloheximide or rapamycin modify cells pleiotropically, the major effect of prolonged growth in lactate was shown to be external pH alkalinization. Importantly, employment of specific inactive Jen1p versions showed that Bul1p-dependent endocytosis requires lactate transport, according to the signal imposed. Our results support a model where conformational changes of Jen1p, associated with substrate/H<sup>+</sup>
 symport, are critical for the efficiency of Bul1p-dependent Jen1p turnover.</div>
</front>
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<Month>11</Month>
<Day>28</Day>
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<DateRevised><Year>2019</Year>
<Month>02</Month>
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<Month>11</Month>
<Day>24</Day>
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<Title>Journal of molecular biology</Title>
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<ArticleTitle>The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals.</ArticleTitle>
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<Abstract><AbstractText>Eukaryotic α-arrestins connect environmental or stress signaling pathways to the endocytosis of plasma membrane transporters or receptors. The Saccharomyces cerevisiae lactate transporter Jen1p has been used as a model cargo for elucidating the mechanisms underlying endocytic turnover in response to carbon sources. Here, we discover a novel pathway of Jen1p endocytosis mediated by the α-arrestin Bul1p in response to the presence of cycloheximide or rapamycin, or prolonged growth in lactate. While cycloheximide or rapamycin modify cells pleiotropically, the major effect of prolonged growth in lactate was shown to be external pH alkalinization. Importantly, employment of specific inactive Jen1p versions showed that Bul1p-dependent endocytosis requires lactate transport, according to the signal imposed. Our results support a model where conformational changes of Jen1p, associated with substrate/H<sup>+</sup>
 symport, are critical for the efficiency of Bul1p-dependent Jen1p turnover.</AbstractText>
<CopyrightInformation>Copyright © 2017 Elsevier Ltd. All rights reserved.</CopyrightInformation>
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<Author ValidYN="Y"><LastName>André</LastName>
<ForeName>Bruno</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Molecular Physiology of the Cell, Université Libre de Bruxelles (ULB), IBMM, Gosselies, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Diallinas</LastName>
<ForeName>George</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Department of Biology, National and Kapodistrian University of Athens, Panepistimioupolis 15784, Athens, Greece.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Paiva</LastName>
<ForeName>Sandra</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus of Gualtar, Braga 4710-057, Portugal. Electronic address: spaiva@bio.uminho.pt.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>09</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Mol Biol</MedlineTA>
<NlmUniqueID>2985088R</NlmUniqueID>
<ISSNLinking>0022-2836</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000468">Alkalies</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019393">Arrestin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099939">BUL1 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C118697">JEN1 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D027501">Monocarboxylic Acid Transporters</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D027981">Symporters</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>147682-31-3</RegistryNumber>
<NameOfSubstance UI="C066958">NPR1 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.3.2.27</RegistryNumber>
<NameOfSubstance UI="D044767">Ubiquitin-Protein Ligases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.-</RegistryNumber>
<NameOfSubstance UI="D011494">Protein Kinases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.3.16</RegistryNumber>
<NameOfSubstance UI="D054648">Protein Phosphatase 2</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.3.16</RegistryNumber>
<NameOfSubstance UI="C104343">SIT4 protein, S cerevisiae</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000468" MajorTopicYN="N">Alkalies</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019393" MajorTopicYN="N">Arrestin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001692" MajorTopicYN="N">Biological Transport</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004705" MajorTopicYN="N">Endocytosis</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053858" MajorTopicYN="N">Metabolic Networks and Pathways</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D027501" MajorTopicYN="N">Monocarboxylic Acid Transporters</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011494" MajorTopicYN="N">Protein Kinases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054648" MajorTopicYN="N">Protein Phosphatase 2</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D027981" MajorTopicYN="N">Symporters</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D044767" MajorTopicYN="N">Ubiquitin-Protein Ligases</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Bul1p</Keyword>
<Keyword MajorTopicYN="Y">Jen1p</Keyword>
<Keyword MajorTopicYN="Y">endocytosis</Keyword>
<Keyword MajorTopicYN="Y">lactate transporter</Keyword>
<Keyword MajorTopicYN="Y">α-arrestin</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>08</Month>
<Day>16</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2017</Year>
<Month>09</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>09</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>10</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>11</Month>
<Day>29</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>10</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28965784</ArticleId>
<ArticleId IdType="pii">S0022-2836(17)30451-5</ArticleId>
<ArticleId IdType="doi">10.1016/j.jmb.2017.09.014</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
<li>Grèce</li>
<li>Portugal</li>
</country>
<region><li>Attique (région)</li>
<li>Région Nord (Portugal)</li>
<li>Région de Bruxelles-Capitale</li>
</region>
<settlement><li>Athènes</li>
<li>Braga</li>
<li>Bruxelles</li>
</settlement>
<orgName><li>Université du Minho</li>
<li>Université libre de Bruxelles</li>
</orgName>
</list>
<tree><country name="Portugal"><region name="Région Nord (Portugal)"><name sortKey="Talaia, Gabriel" sort="Talaia, Gabriel" uniqKey="Talaia G" first="Gabriel" last="Talaia">Gabriel Talaia</name>
</region>
<name sortKey="Barata Antunes, Claudia" sort="Barata Antunes, Claudia" uniqKey="Barata Antunes C" first="Cláudia" last="Barata-Antunes">Cláudia Barata-Antunes</name>
<name sortKey="Casal, Margarida" sort="Casal, Margarida" uniqKey="Casal M" first="Margarida" last="Casal">Margarida Casal</name>
<name sortKey="Paiva, Sandra" sort="Paiva, Sandra" uniqKey="Paiva S" first="Sandra" last="Paiva">Sandra Paiva</name>
</country>
<country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Gournas, Christos" sort="Gournas, Christos" uniqKey="Gournas C" first="Christos" last="Gournas">Christos Gournas</name>
</region>
<name sortKey="Andre, Bruno" sort="Andre, Bruno" uniqKey="Andre B" first="Bruno" last="André">Bruno André</name>
<name sortKey="Saliba, Elie" sort="Saliba, Elie" uniqKey="Saliba E" first="Elie" last="Saliba">Elie Saliba</name>
</country>
<country name="Grèce"><region name="Attique (région)"><name sortKey="Diallinas, George" sort="Diallinas, George" uniqKey="Diallinas G" first="George" last="Diallinas">George Diallinas</name>
</region>
</country>
</tree>
</affiliations>
</record>

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	Serveur d'exploration sur la rapamycine et les champignons
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The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals.

The α-Arrestin Bul1p Mediates Lactate Transporter Endocytosis in Response to Alkalinization and Distinct Physiological Signals.

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